| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Czirjak, Sara E (12)
|
VAGATR |
33.59 |
33.42 |
-0.17 |
| 2 |
Ismailova, Diana (11)
|
VAGATR |
34.98 |
35.46 |
0.48 |
| 3 |
Rogerson, Annika R (12)
|
VAUN-V |
40.28 |
39.88 |
-0.40 |
| 4 |
Dugas, Georgia K (12)
|
VAACAC |
46.92 |
40.00 |
-6.92 |
| 5 |
Hoang, Angelina (11)
|
VAACAC |
|
40.92 |
- |
| 6 |
McCardle, Reese B (11)
|
VAACAC |
42.40 |
41.68 |
-0.72 |
| 7 |
Segura, Mara J (11)
|
VATSU |
47.64 |
44.71 |
-2.93 |
| 8 |
Turner, Paige Y (11)
|
VATSU |
54.37 |
46.69 |
-7.68 |
| 9 |
Brooks, Madelyne G (11)
|
VABASS |
50.15 |
47.60 |
-2.55 |
| 10 |
Brushwood, Madison P (11)
|
VABASS |
48.75 |
48.09 |
-0.66 |
| 11 |
Wells, Abigail J (11)
|
VATSU |
1:10.88 |
48.75 |
-22.13 |
| 12 |
Hernandez, Maya G (11)
|
VABASS |
|
49.46 |
- |
| 13 |
Barclay, Sydney M (12)
|
VAACAC |
49.75 |
49.51 |
-0.24 |
| 14 |
Semmelhack, Sylvie W (11)
|
VAGATR |
|
51.84 |
- |
| 15 |
Brice, Madison R (11)
|
VABASS |
|
1:01.47 |
- |
|
Mejeur, Hannah J (12)
|
VATSU |
|
DQ |
- |
|
McCORKLE, Madeline G (11)
|
VATSU |
|
DQ |
- |
|
Martin, Grace E (11)
|
VABASS |
|
DQ |
- |